c-Rel | HUABIO ET1705-44 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

c-Rel

catalog :

ET1705-44

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM72-93

reactivity :

human

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of c-Rel on different lysates with Rabbit anti-c-Rel antibody (ET1705-44) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: Raji cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: U-937 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 69 kDa Observed band size: 75 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-44) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of c-Rel on different lysates with Rabbit anti-c-Rel antibody (ET1705-44) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-c-Rel KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 69 kDa Observed band size: 75 kDa Exposure time: 1 minute 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-44) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-c-Rel antibody. Counter stained with hematoxylin.

product information

SKU :

ET1705-44

Target name :

c-Rel

Species reactivity :

Human

Applications :

WB,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human c-Rel aa 501-550 / 619.

Uniprot id :

Q04864>SwissProt: Q04864 Human

Host :

Rabbit

Clone number :

JM72-93

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, K-562 cell lysate, Raji cell lysate, Daudi cell lysate, Jurkat cell lysate, A431 cell lysate, U-937 cell lysate, human tonsil tissue, human colon cancer tissue, human skin tissue, human placenta tissue.

Molecular wt :

Predicted band size: 69 kDa

Subcellular location :

Cytoplasm. Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1705-44_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-c-Rel antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1705-44_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-c-Rel antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1705-44_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-c-Rel antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage