IKB epsilon | HUABIO ET1705-40 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

IKB epsilon

catalog :

ET1705-40

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM62-63

reactivity :

human

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of IKB epsilon on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-40, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate Predicted band size: 53 kDa Observed band size: 40 kDa

image 2 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IKB epsilon antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-40, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1705-40

Target name :

IKB epsilon

Species reactivity :

Human

Applications :

WB,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human IKB epsilon aa 175-224 / 500.

Uniprot id :

O00221>SwissProt: O00221 Human

Host :

Rabbit

Clone number :

JM62-63

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

THP-1 cell lysate, Jurkat cell lysate, Hela cell lysate, human tonsil tissue.

Molecular wt :

Predicted band size: 53 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IHC-P: 1:50-1:200

more info or order :

HUABIO product webpage