DDX5 | HUABIO ET1705-32 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

DDX5

catalog :

ET1705-32

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM32-50

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of DDX5 on different lysates with Rabbit anti-DDX5 antibody (ET1705-32) at 1/500 dilution. Lane 1: PC-12 cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-32) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of DDX5 on different lysates with Rabbit anti-DDX5 antibody (ET1705-32) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: SH-SY5Y cell lysate (10 µg/Lane) Lysates/proteins at 20 µg/Lane. Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-32) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of DDX5 on different lysates with Rabbit anti-DDX5 antibody (ET1705-32) at 1/500 dilution. Lane 1: Hela cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 2 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-32) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1705-32

Target name :

DDX5

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human DDX5 aa 1-50 / 614.

Uniprot id :

P17844>SwissProt: P17844 Human;SwissProt: Q61656 Mouse;Entrez Gene: 287765 Rat

Host :

Rabbit

Clone number :

JM32-50

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

PC-12 cell lysate, NIH/3T3 cell lysate, Hela, SH-SY5Y, SW480, rat brain tissue, human placenta tissue, mouse brain tissue, mouse brain tissue lysate, SH-SY5Y cell lysate, human colon carcinoma tissue, mouse hippocampus tissue.

Molecular wt :

Predicted band size: 69 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:2,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1705-32_4.jpg

Pic legend4 :

Immunocytochemistry analysis of NIH/3T3 cells labeling DDX5 with Rabbit anti-DDX5 antibody (ET1705-32) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DDX5 antibody (ET1705-32) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1705-32_5.jpg

Pic legend5 :

Immunocytochemistry analysis of PC-12 cells labeling DDX5 with Rabbit anti-DDX5 antibody (ET1705-32) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DDX5 antibody (ET1705-32) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1705-32_6.jpg

Pic legend6 :

Immunocytochemistry analysis of SH-SY5Y cells labeling DDX5 with Rabbit anti-DDX5 antibody (ET1705-32) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DDX5 antibody (ET1705-32) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1705-32_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-DDX5 antibody (ET1705-32) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-32) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1705-32_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-DDX5 antibody (ET1705-32) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-32) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1705-32_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-DDX5 antibody (ET1705-32) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-32) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1705-32_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-DDX5 antibody (ET1705-32) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-32) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ET1705-32_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-DDX5 antibody (ET1705-32) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-32) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage