Placental alkaline phosphatase | HUABIO ET1705-21 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Placental alkaline phosphatase

catalog :

ET1705-21

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM22-53

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Placental alkaline phosphatase on different lysates with Rabbit anti-Placental alkaline phosphatase antibody (ET1705-21) at 1/5,000 dilution. Lane 1: A431 cell lysate Lane 2: SiHa cell lysate Lane 3: AGS cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 1 minute 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-21) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Placental alkaline phosphatase on human placenta tissue lysates with Rabbit anti-Placental alkaline phosphatase antibody (ET1705-21) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-21) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Placental alkaline phosphatase antibody (ET1705-21) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-21) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1705-21

Target name :

Placental alkaline phosphatase

Species reactivity :

Human

Applications :

WB,IF-Tissue,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Placental alkaline phosphatase aa 171-220 / 535.

Uniprot id :

P05187>SwissProt: P05187 Human

Host :

Rabbit

Clone number :

JM22-53

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 cell lysate, SiHa cell lysate, AGS cell lysate, human placenta tissue lysates, human placenta tissue, human seminoma tissue.

Molecular wt :

Predicted band size: 58 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:5,000 ;IF-Tissue: 1:500 ;IHC-P: 1:50-1:400

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1705-21_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human seminoma tissue with Rabbit anti-Placental alkaline phosphatase antibody (ET1705-21) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-21) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1705-21_5.jpg

Pic legend5 :

Application: IF-Tissue Species: Human Site: placenta Sample: Paraffin-embedded section Antibody concentration: 1/500

more info or order :

HUABIO product webpage