TAK1 | HUABIO ET1705-14 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TAK1

catalog :

ET1705-14

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM73-19

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TAK1 on different lysates with Rabbit anti-TAK1 antibody (ET1705-14) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD TAK1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 67 kDa Observed band size: 72 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-14) at 1/1,000 dilution was used in 2% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of TAK1 on different lysates with Rabbit anti-TAK1 antibody (ET1705-14) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: Mouse lung tissue lysate (40 µg/Lane) Lane 6: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 67 kDa Observed band size: 72 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of TAK1 on different lysates with Rabbit anti-TAK1 antibody (ET1705-14) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1705-14

Target name :

TAK1

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within C-terminal human TAK1.

Uniprot id :

O43318>SwissProt: O43318 Human;SwissProt: Q62073 Mouse;SwissProt: P0C8E4 Rat

Host :

Rabbit

Clone number :

JM73-19

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse lung tissue lysate, Rat lung tissue lysate, HeLa cell lysate, COS-1 cell lysate.

Molecular wt :

Predicted band size: 67 kDa

Subcellular location :

Cytoplasm, Cell membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000

Advanced Validation :

Knockdown (KD)

more info or order :

HUABIO product webpage