Anti-Glucose Transporter GLUT3 Antibody [JA50-31] | HUABIO ET1704-59TR product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Anti-Glucose Transporter GLUT3 Antibody [JA50-31]

catalog :

ET1704-59TR

quantity :

20 uL

price :

99 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JA50-31

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

product information

SKU :

ET1704-59TR

Target name :

Anti-Glucose Transporter GLUT3 Antibody [JA50-31]

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human GLUT3 aa 11-60 / 496.

Uniprot id :

SwissProt: P11169 Human;SwissProt: P32037 Mouse;SwissProt: Q07647 Rat

Host :

Rabbit

Clone number :

JA50-31

Isotype :

IgG

Size :

20 uL

List Price :

99 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2 cell lysate, RAW264.7 cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human brain tissue, human embryo tissue, mouse brain tissue, SH-SY5Y.

Molecular wt :

Predicted band size: 54 kDa

Subcellular location :

Cell membrane. Perikaryon. Cell projection.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Pic img4 :

ET1704-59_2.jpg

Pic img5 :

Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Glucose Transporter GLUT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic legend5 :

ET1704-59_3.jpg

Pic legend6 :

Western blot analysis of Glucose Transporter GLUT3 on different lysates with Rabbit anti-Glucose Transporter GLUT3 antibody (ET1704-59) at 1/1,000 dilution. Lane 1: HepG2-si NT cell lysate (10 µg/Lane) Lane 2: HepG2-si Glucose Transporter GLUT3 cell lysate (10 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute; ECL: merk 4-20% SDS-PAGE gel. ET1704-59 was shown to specifically react with Glucose Transporter GLUT3 in HepG2-si NT cells. Weakened band was observed when HepG2-si Glucose Transporter GLUT3 sample was tested. HepG2-si NT and HepG2-si Glucose Transporter GLUT3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1704-59, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Pic img7 :

ET1704-59_4.jpg

Pic img8 :

Immunohistochemical analysis of paraffin-embedded human embryo tissue using anti-Glucose Transporter GLUT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic legend8 :

ET1704-59_5.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Glucose Transporter GLUT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

ET1704-59_6.jpg

Pic img11 :

Flow cytometric analysis of Glucose Transporter GLUT3 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-59, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage