Western blot analysis of Glucose Transporter GLUT3 on different lysates with Rabbit anti-Glucose Transporter GLUT3 antibody (ET1704-59) at 1/1,000 dilution. Lane 1: HepG2-si NT cell lysate (10 µg/Lane) Lane 2: HepG2-si Glucose Transporter GLUT3 cell lysate (10 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute; ECL: merk 4-20% SDS-PAGE gel. ET1704-59 was shown to specifically react with Glucose Transporter GLUT3 in HepG2-si NT cells. Weakened band was observed when HepG2-si Glucose Transporter GLUT3 sample was tested. HepG2-si NT and HepG2-si Glucose Transporter GLUT3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1704-59, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of Glucose Transporter GLUT3 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-59, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).