antibody

Anti-Leptin Receptor Antibody [JA73-01]

ET1704-44TR

20 uL

99 USD

monoclonal

domestic rabbit

nonconjugated

JA73-01

human, mouse, rat

western blot, flow cytometry, immunohistochemistry - paraffin section

Anti-Leptin Receptor Antibody [JA73-01]

Human,Mouse,Rat

WB,IF-Cell,IHC-P,FC

Non-conjugated

Synthetic peptide within Human Leptin Receptor aa 1041-1090 / 1165.

SwissProt: P48357 Human;SwissProt: P48356 Mouse;SwissProt: Q62959 Rat

Rabbit

JA73-01

IgG

20 uL

99 USD

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Liquid

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Protein A affinity purified.

Recombinant Rabbit monoclonal Antibody

Mouse lung tissue lysate, mouse liver tissue lysate, K-562 cell lysates, HeLa cell lysates, rat brain tissue lysates, MCF-7, rat heart tissue, human liver carcinoma tissue, human prostate tissue, human kidney tissue, mouse small intestine tissue, K562.

Predicted band size: 132 kDa

Cell membrane, Basolateral cell membrane.

1 mg/mL.

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:100 ;IHC-P: 1:50-1:1,000 ;FC: 1:50-1:100

ET1704-44_2.jpg

Western blot analysis of Leptin Receptor on different lysates with Rabbit anti-Leptin Receptor antibody (ET1704-44) at 1/1,000 dilution. Lane 1: K-562 cell lysate (16 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (32 µg/Lane) Predicted band size: 132 kDa Observed band size: 132 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-44) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

ET1704-44_3.jpg

ICC staining of Leptin Receptor in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-44, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ET1704-44_4.jpg

Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Leptin Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1704-44_5.jpg

Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Leptin Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1704-44_6.jpg

Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Leptin Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1704-44_7.jpg

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Leptin Receptor antibody (ET1704-44) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-44) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1704-44_8.jpg

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Leptin Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1704-44_9.jpg

Flow cytometric analysis of Leptin Receptor was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-44, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).