product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
alpha sarcoglycan
catalog :
ET1704-25
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JA51-81
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of alpha sarcoglycan on different lysates with Rabbit anti-alpha sarcoglycan antibody (ET1704-25) at 1/1,000 dilution.
Lane 1: Mouse heart tissue lysate
Lane 2: Mouse skeletal muscle tissue lysate
Lane 3: Rat heart tissue lysate
Lane 4: Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 50 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-25) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of alpha sarcoglycan on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-25, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: mouse heart tissue lysate
Lane 2: human skeletal muscle tissue lysate
image 3 :
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-alpha sarcoglycan antibody (ET1704-25) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-25) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
ET1704-25
Target name :
alpha sarcoglycan
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IP,IHC-P,IF-Tissue
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human alpha sarcoglycan aa 338-387 / 387.
Uniprot id :
Q16586>SwissProt: Q16586 Human;SwissProt: P82350 Mouse;SwissProt: Q5SWB2 Rat
Host :
Rabbit
Clone number :
JA51-81
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Mouse heart tissue lysate, Mouse skeletal muscle tissue lysate, Rat heart tissue lysate, Rat skeletal muscle tissue lysate, mouse heart tissue lysate, human skeletal muscle tissue lysate, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, mouse heart tissue, rat heart tissue.
Molecular wt :
Predicted band size: 43 kDa
Subcellular location :
Sarcolemma, cytoskeleton.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IHC-P: 1:200-1:1,000
;IP: 1-2μg/sample
;IF-Tissue: 1:200
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET1704-25_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-alpha sarcoglycan antibody (ET1704-25) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-25) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET1704-25_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-alpha sarcoglycan antibody (ET1704-25) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-25) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ET1704-25_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-alpha sarcoglycan antibody (ET1704-25) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-25) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/ET1704-25_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-alpha sarcoglycan antibody (ET1704-25) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-25) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/ET1704-25_8.jpg
Pic legend8 :
alpha sarcoglycan was immunoprecipitated from 0.2 mg mouse heart tissue lysate with ET1704-25 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1704-25 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse heart tissue lysate (input)
Lane 2: ET1704-25 IP in mouse heart tissue lysate
Lane 3: Rabbit IgG instead of ET1704-25 in mouse heart tissue lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 30 seconds; ECL: K1801
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
browse more products
questions and comments