product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
Phosphotyrosine
catalog :
ET1704-20
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JA10-49
application :
western blot, immunoprecipitation, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of Phosphotyrosine on A431 cells lysates treated (Line 1) and untreated with Sodium metavanadate using anti- Phosphotyrosine antibody at 1/100 dilution.
image 2 :
ICC staining Phosphotyrosine in A431 cells (green) nontreated (Left) or treated with Sodium metavanadate (Right). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
image 3 :
Flow cytometric analysis of A431 cells with Phosphotyrosine antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
product information
SKU :
ET1704-20
Target name :
Phosphotyrosine
Species reactivity :
Species independent
Applications :
WB,IF-Cell,IF-Tissue,IP,FC
Conjugate :
Non-conjugated
Immunogen :
CXXXX-pY-XXXXNH2.
Uniprot id :
>
Host :
Rabbit
Clone number :
JA10-49
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
A431 cells lysates treated with Sodium metavanadate.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:50-1:200
;FC: 1:10-1:50
;IF-Cell: 1:50
;IF-Tissue: 1:50
Advanced Validation :
Cell treatment (CT)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
browse more products
questions and comments