product summary
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company name :
HUABIO
product type :
antibody
product name :
Vitamin D Receptor
catalog :
ET1704-09
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JA11-16
reactivity :
human, mouse, rat
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Vitamin D Receptor on different lysates with Rabbit anti-Vitamin D Receptor antibody (ET1704-09) at 1/5,000 dilution.
Lane 1: HL-60 cell lysate (15 µg/Lane)
Lane 2: T-47D cell lysate (15 µg/Lane)
Lane 3: NIH/3T3 cell lysate (15 µg/Lane)
Lane 4: L-929 cell lysate (15 µg/Lane)
Lane 5: Rat kidney tissue lysate (20 µg/Lane)
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-09) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
All lanes: Western blot analysis of Vitamin D Receptor with anti-Vitamin D Receptor antibody[JA11-16] (ET1704-09) at 1:500 dilution.
Lane 1: Wild-type Hela whole cell lysate (10 µg).
Lane 2/3: Vitamin D Receptor knockout Hela whole cell lysate (10 µg).
ET1704-09 was shown to specifically react with Vitamin D Receptor in wild-type Hela cells. No bands were observed when Vitamin D Receptor knockout sample were tested. Wild-type and Vitamin D Receptor knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1704-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
image 3 :
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-09, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
ET1704-09
Target name :
Vitamin D Receptor
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide with Human Vitamin D Receptor aa 1-50 / 427.
Uniprot id :
P11473>SwissProt: P11473 Human;SwissProt: P48281 Mouse;SwissProt: P13053 Rat
Host :
Rabbit
Clone number :
JA11-16
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HL-60 cell lysate, T-47D cell lysate, NIH/3T3 cell lysate, L-929 cell lysate, Hela WT cell lysate, rat large intestine tissue, human skin tissue, mouse colon tissue, mouse skin tissue, Hela, rat skin tissue.
Molecular wt :
Predicted band size: 48 kDa
Subcellular location :
Nucleus.
Concentration :
1mg/ml.
Recommended dilutions :
WB: 1:5,000
;IHC-P: 1:50-1:200
;FC: 1:50-1:100
Advanced Validation :
Knockout (KO)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET1704-09_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-09, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET1704-09_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Vitamin D Receptor antibody (ET1704-09) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-09) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ET1704-09_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-09, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/ET1704-09_7.jpg
Pic legend7 :
Flow cytometric analysis of Vitamin D Receptor was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-09, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Pic img8 :
https://storage.huabio.cn/huabio/productImg/ET1704-09_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Vitamin D Receptor antibody (ET1704-09) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-09) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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