product summary
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company name :
HUABIO
product type :
antibody
product name :
MINA53
catalog :
ET1704-07
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JA11-63
reactivity :
human, mouse
application :
western blot, immunoprecipitation
more info or order :
image
image 1 :
Western blot analysis of MINA53 on different lysates with Rabbit anti-MINA53 antibody (ET1704-07) at 1/2,000 dilution.
Lane 1: HDLM-2 cell lysate
Lane 2: HUT 102 cell lysate
Lane 3: KG-1 cell lysate
Lane 4: A431 cell lysate
Lane 5: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-07) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of MINA53 on different lysates with Rabbit anti-MINA53 antibody (ET1704-07) at 1/2,000 dilution.
Lane 1: Mouse testis tissue lysate
Lane 2: Mouse placenta (E16.5) tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 13 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-07) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
MINA53 was immunoprecipitated from 0.2 mg A549 cell lysate with ET1704-07 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1704-07 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate (input)
Lane 2: ET1704-07 IP in A549 cell lysate
Lane 3: Rabbit IgG instead of ET1704-07 in A549 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 13 seconds; ECL: K1801
product information
SKU :
ET1704-07
Target name :
MINA53
Species reactivity :
Human,Mouse
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human MINA53 aa 416-465 / 465.
Uniprot id :
Q8IUF8>SwissProt: Q8IUF8 Human;SwissProt: Q8CD15 Mouse
Host :
Rabbit
Clone number :
JA11-63
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HDLM-2 cell lysate, HUT 102 cell lysate, KG-1 cell lysate, A431 cell lysate, A549 cell lysate, Mouse testis tissue lysate, Mouse placenta (E16.5) tissue lysate.
Molecular wt :
Predicted band size: 53 kDa
Subcellular location :
Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IP: 1-2μg/sample
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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