MIF | HUABIO ET1703-89 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MIF

catalog :

ET1703-89

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM11-64

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MIF on different lysates with Rabbit anti-MIF antibody (ET1703-89) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-MIF KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-89) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of MIF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse brain tissue lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate

image 3 :

Flow cytometric analysis of MIF was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-89, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

ET1703-89

Target name :

MIF

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human MIF aa 60-90.

Uniprot id :

P14174>SwissProt: P14174 Human;SwissProt: P34884 Mouse;SwissProt: P30904 Rat

Host :

Rabbit

Clone number :

JM11-64

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse brain tissue lysate, Jurkat cell lysate, Hela cell lysate, THP-1.

Molecular wt :

Predicted band size: 12 kDa

Subcellular location :

Secreted. Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IP: 1:10-1:50 ;FC: 1:50-1:100

Advanced Validation :

Knockdown (KD)

more info or order :

HUABIO product webpage