product summary
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company name :
HUABIO
product type :
antibody
product name :
CRISPR-Cas9 SP
catalog :
ET1703-85-50UL
quantity :
50μl
price :
205.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JM11-55
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of CRISPR-Cas9 SP on 293T transfected with Cas9 cell lysates with Rabbit anti-CRISPR-Cas9 SP antibody (ET1703-85) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 158 kDa
Observed band size: 158 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-85) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
ET1703-85-50UL
Target name :
CRISPR-Cas9 SP
Species reactivity :
Streptococcus pyogenes
Applications :
WB,IF-Cell,IF-Tissue,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Streptococcus pyogenes cas9 aa 730-990 / 1368.
Uniprot id :
Q99ZW2>SwissProt: Q99ZW2 StreptococcusPyogenes
Host :
Rabbit
Clone number :
JM11-55
Isotype :
IgG
Size :
50μl
List Price :
205.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
293T transfected with Cas9 cell lysates.
Molecular wt :
158 kDa
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:5,000
;IF-Cell: 1:50-1:200
;IF-Tissue: 1:50-1:200
;IHC-P: 1:50-1:200
;FC: 1:50-1:100
Advanced Validation :
Cell treatment (CT)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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