NMDAR1 | HUABIO ET1703-75-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NMDAR1

catalog :

ET1703-75-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM11-26

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 3 :

Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

product information

SKU :

ET1703-75-50UL

Target name :

NMDAR1

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IF-Tissue,IHC-P,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human NMDAR1 aa 870-910.

Uniprot id :

Q05586>SwissProt: Q05586 Human;SwissProt: P35438 Mouse;SwissProt: P35439 Rat

Host :

Rabbit

Clone number :

JM11-26

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MCF7 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, N2A, SHG-44, SH-SY5Y, mouse cerebral cortex tissue, rat cerebral cortex tissue, mouse hippocampus tissue, mouse cerebral cortex tissue, rat cerebral cortex tissue.

Molecular wt :

Predicted band size: 105 kDa

Subcellular location :

Cell membrane, postsynaptic cell membrane, postsynaptic density.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Tissue: 1:200-1:500 ;IHC-P: 1:1,000 ;IHC-Fr: 1:500

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1703-75_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue with Rabbit anti-NMDAR1 antibody (ET1703-75) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-75) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1703-75_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue with Rabbit anti-NMDAR1 antibody (ET1703-75) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-75) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1703-75_6.jpg

Pic legend6 :

Western blot analysis of NMDAR1 on different lysates with Rabbit anti-NMDAR1 antibody (ET1703-75) at 1/5,000 dilution. Lane 1: MCF7 cell lysate (15 µg/Lane) Lane 2: Human brain tissue lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Lane 5: Mouse heart tissue lysate (negative) (20 µg/Lane) Lane 6: Rat liver tissue lysate (negative) (20 µg/Lane) Predicted band size: 105 kDa Observed band size: 120 kDa Exposure time: 1 minute 2 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-75) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1703-75_7.jpg

Pic legend7 :

Application: IF-tissue Species: Mouse Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1:200

more info or order :

HUABIO product webpage