TDP43 | HUABIO ET1703-74-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TDP43

catalog :

ET1703-74-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM51-10

reactivity :

human, mouse, zebrafish

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TDP43 on different lysates with Rabbit anti-TDP43 antibody (ET1703-74) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: Neuro-2a cell lysate Lane 4: Mouse spleen tissue lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat spleen tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-74) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

image 3 :

Immunocytochemistry analysis of HeLa cells labeling TDP43 with Rabbit anti-TDP43 antibody (ET1703-74) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TDP43 antibody (ET1703-74) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

ET1703-74-50UL

Target name :

TDP43

Species reactivity :

Human,Mouse,Rat,Zebrafish,Cynomolgus monkey,Pig

Applications :

WB,IP,IF-Cell,IF-Tissue,IHC-P,FC,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human TDP43 aa 354-390 / 414; (2)aa 253-290 / 414.

Uniprot id :

Q13148>SwissProt: Q13148 Human;SwissProt: Q921F2 Mouse;Entrez Gene: 298648 Rat;Entrez Gene:325052 Zebrafish

Host :

Rabbit

Clone number :

JM51-10

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, K-562 cell lysate, Neuro-2a cell lysate, mouse spleen tissue lysate, mouse brain tissue lysate, rat spleen tissue lysate, rat brain tissue lysate, HeLa, Neuro-2a, human spleen tissue, mouse placenta tissue, human pancreas tissue, mouse hippocampus tissue, mouse cerebral cortex tissue, mouse cerebrum tissue.

Molecular wt :

Predicted band size: 45 kDa

Subcellular location :

Cytoplasm, Mitochondrion, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IHC-Fr: 1:200-1:500 ;IF-Cell: 1:250-1:500 ;IF-Tissue: 1:100 ;IHC-P: 1:500 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1703-74_4.jpg

Pic legend4 :

Immunocytochemistry analysis of Neuro-2a cells labeling TDP43 with Rabbit anti-TDP43 antibody (ET1703-74) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TDP43 antibody (ET1703-74) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1703-74_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-TDP43 antibody (ET1703-74) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-74) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1703-74_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-TDP43 antibody (ET1703-74) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-74) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1703-74_7.jpg

Pic legend7 :

Flow cytometric analysis of TDP43 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-74, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage