PDIA3 / ERp57 | HUABIO ET1703-68 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PDIA3 / ERp57

catalog :

ET1703-68

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM11-44

reactivity :

human

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PDIA3 / ERp57 on different lysates with Rabbit anti-PDIA3 / ERp57 antibody (ET1703-68) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: LNCaP cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-68) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of PDIA3 / ERp57 on different lysates with Rabbit anti-PDIA3 / ERp57 antibody (ET1703-68) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-PDIA3 / ERp57 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 57 kDa Observed band size: 50 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-68) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ERp57 antibody. Counter stained with hematoxylin.

product information

SKU :

ET1703-68

Target name :

PDIA3 / ERp57

Species reactivity :

Human

Applications :

WB,IP,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human PDIA3 aa 175-208 / 505.

Uniprot id :

P30101>SwissProt: P30101 Human

Host :

Rabbit

Clone number :

JM11-44

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2 cell lysate, LNCaP cell lysate, human kidney tissue, human pancreas tissue, human uterus tissue.

Molecular wt :

Predicted band size: 57 kDa

Subcellular location :

Endoplasmic reticulum lumen. Melanosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:50-1:200 ;IP: 1:10-1:50

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1703-68_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ERp57 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1703-68_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-ERp57 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage