FGFR3 | HUABIO ET1703-65-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

FGFR3

catalog :

ET1703-65-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM110-33

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of FGFR3 on different lysates with Rabbit anti-FGFR3 antibody (ET1703-65) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: 293 cell lysate Lane 3: MCF-7 cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 88 kDa Observed band size: 95 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-65) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of FGFR3 on Rat skin tissue lysates with Rabbit anti-FGFR3 antibody (ET1703-65) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 95 kDa Exposure time: 8 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-65) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:10,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of FGFR3 on Mouse hippocampus lysates with Rabbit anti-FGFR3 antibody (ET1703-65) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 95 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-65) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1703-65-50UL

Target name :

FGFR3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human FGFR3 aa 30-63 / 806.

Uniprot id :

P22607>SwissProt: P22607 Human;SwissProt: Q61851 Mouse;Unigene:23671 Rat

Host :

Rabbit

Clone number :

JM110-33

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Hela cell lysate, 293 cell lysate, MCF-7 cell lysate, HepG2 cell lysate, Rat skin tissue lysates, Mouse hippocampus lysates, MCF-7, HepG2, SH-SY5Y, human kidney tissue, mouse testis tissue, mouse kidney tissue, mouse brain tissue.

Molecular wt :

Predicted band size: 88 kDa

Subcellular location :

Cell membrane, Endoplasmic reticulum, Cytoplasmic vesicle, Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:500-1:2,000 ;IHC-P: 1:10,000-1:20,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1703-65_4.jpg

Pic legend4 :

Western blot analysis of FGFR3 on different lysates with Rabbit anti-FGFR3 antibody (ET1703-65) at 1/1,000 dilution. Lane 1: HEK293-si NT cell lysate Lane 2: HEK293-si FGFR3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 88 kDa Observed band size: 130 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. ET1703-65 was shown to specifically react with FGFR3 in HEK293-si NT cells. Weakened band was observed when HEK293-si FGFR3 sample was tested. HEK293-si NT and HEK293-si FGFR3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1703-65, 1/1,000) and Loading control antibody (Rabbit anti-HSP90, ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1703-65_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue with Rabbit anti-FGFR3 antibody (ET1703-65) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-65) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1703-65_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-FGFR3 antibody (ET1703-65) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-65) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1703-65_7.jpg

Pic legend7 :

ICC staining of FGFR3 in MCF-7 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-65, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

more info or order :

HUABIO product webpage