ADAM10 | HUABIO ET1703-60 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ADAM10

catalog :

ET1703-60

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM32-11

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ADAM10 on different lysates with Rabbit anti-ADAM10 antibody (ET1703-60) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: Human lung tissue lysate (30 µg/Lane) Lane 4: RAW264.7 cell lysate (15 µg/Lane) Lane 5: Mouse placenta tissue lysate (30 µg/Lane) Lane 6: Mouse brain tissue lysate (30 µg/Lane) Lane 7: Rat brain tissue lysate (30 µg/Lane) Lane 8: Rat spleen tissue lysate (30 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 84 kDa Observed band size: 75/100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-60) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of ADAM10 on different lysates with Rabbit anti-ADAM10 antibody (ET1703-60) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si ADAM10 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 84 kDa Observed band size: 84/70 kDa Exposure time: 2 minutes ; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-60) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-ADAM10 antibody (ET1703-60) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-60) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1703-60

Target name :

ADAM10

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human ADAM10 aa 715-748 / 748.

Uniprot id :

O14672>SwissProt: O14672 Human;SwissProt: O35598 Mouse;SwissProt: Q10743 Rat

Host :

Rabbit

Clone number :

JM32-11

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysate, HeLa cell lysate, human lung tissue lysate, RAW264.7 cell lysate, mouse placenta tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, rat spleen tissue lysate, human thyroid carcinoma tissue, human stomach tissue.

Molecular wt :

Predicted band size: 84 kDa

Subcellular location :

Cell membrane. Endomembrane system.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:1,000 ;IP: 1-2μg/sample

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1703-60_4.jpg

Pic legend4 :

ADAM10 was immunoprecipitated from 0.2 mg HeLa cell lysate with ET1703-60 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1703-60 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: ET1703-60 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of ET1703-60 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 59 seconds; ECL: K1801

more info or order :

HUABIO product webpage