NF-M | HUABIO ET1703-57 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NF-M

catalog :

ET1703-57

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM11-20

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-NF-M antibody (ET1703-57) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-57) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1703-57

Target name :

NF-M

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey

Applications :

WB,IHC-P,IHC-Fr,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human NEFM aa 651-692 / 916.

Uniprot id :

P07197>SwissProt: P07197 Human;SwissProt: P08553 Mouse;SwissProt: P12839 Rat

Host :

Rabbit

Clone number :

JM11-20

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse cerebellum tissue, rat cerebellum tissue, human cerebellum tissue, 293T cell lysate, Rat brain tissue lysate, Mouse brain tissue lysate.

Molecular wt :

Predicted band size: 102 kDa

Subcellular location :

Cell projection, Cytoplasm, Cytoskeleton, Intermediate filament.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:2,000 ;IHC-Fr: 1:500 ;IF-Tissue: 1:500

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1703-57_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-NF-M antibody (ET1703-57) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-57) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1703-57_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-NF-M antibody (ET1703-57) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-57) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1703-57_6.jpg

Pic legend6 :

Western blot analysis of NF-M on different lysates with Rabbit anti-NF-M antibody (ET1703-57) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: Rat brain tissue lysate (40 µg/Lane) Lane 3: Rat liver tissue lysate (negative) (40 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 5: Mouse liver tissue lysate (negative) (40 µg/Lane) Predicted band size: 102 kDa Observed band size: 150 kDa Exposure time: Lane 1-3: 3 minutes; Lane 4-5: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-57) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage