product summary
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company name :
HUABIO
product type :
antibody
product name :
L1CAM
catalog :
ET1703-51
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JM11-05
reactivity :
human
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of L1CAM on different lysates with Rabbit anti-L1CAM antibody (ET1703-51) at 1/1,000 dilution.
Lane 1: A375 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (low expression) (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: Human brain tissue lysate (20 µg/Lane)
Predicted band size: 140 kDa
Observed band size: 250 kDa
Exposure time: 10 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-51) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-L1CAM antibody (ET1703-51) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-L1CAM antibody (ET1703-51) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
ET1703-51
Target name :
L1CAM
Species reactivity :
Human
Applications :
WB,IHC-P,IF-Tissue
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human L1CAM aa 900-1135 / 1257.
Uniprot id :
P32004>SwissProt: P32004 Human
Host :
Rabbit
Clone number :
JM11-05
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
A375 cell lysate, HeLa cell lysate, human brain tissue lysate, human kidney tissue, human appendix tissue, human brain tissue, human colon cancer tissue, human prostate cancer tissue.
Molecular wt :
Predicted band size: 140 kDa
Subcellular location :
Cell membrane, Cell projection, growth cone, axon, dendrite.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:5,000
;IHC-P: 1:200-1:1,000
;IF-Tissue: 1:200
Advanced Validation :
Relative expression (RE)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET1703-51_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-L1CAM antibody (ET1703-51) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET1703-51_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-L1CAM antibody (ET1703-51) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ET1703-51_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-L1CAM antibody (ET1703-51) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/ET1703-51_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-L1CAM antibody (ET1703-51) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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