Anti-Hemoglobin subunit gamma 1and2 Antibody [JM84-10] | HUABIO ET1703-46TR product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Anti-Hemoglobin subunit gamma 1and2 Antibody [JM84-10]

catalog :

ET1703-46TR

quantity :

20 uL

price :

99 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM84-10

reactivity :

human, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

product information

SKU :

ET1703-46TR

Target name :

Anti-Hemoglobin subunit gamma 1and2 Antibody [JM84-10]

Species reactivity :

Human,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human HBG1 / 2 aa 2-45 / 147.

Uniprot id :

SwissProt: P69891 Human;SwissProt: P69892 Human

Host :

Rabbit

Clone number :

JM84-10

Isotype :

IgG

Size :

20 uL

List Price :

99 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human placenta tissue lysate, human brain tissue lysate, human placenta tissue, human stomach tissue (negative).

Molecular wt :

Predicted band size: 16 kDa

Subcellular location :

Cytosol, hemoglobin complex.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Cell: 1:50-1:100 ;IF-Tissue: 1:50-1:100 ;IHC-P: 1:1000

Pic img4 :

ET1703-46_2.jpg

Pic img5 :

ICC staining of Hemoglobin subunit gamma 1and2 in D3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-46, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic legend5 :

ET1703-46_3.jpg

Pic legend6 :

ICC staining of Hemoglobin subunit gamma 1and2 in MCF-7 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-46, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img7 :

ET1703-46_4.jpg

Pic img8 :

ICC staining of Hemoglobin subunit gamma 1and2 in PC-12 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-46, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic legend8 :

ET1703-46_5.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Hemoglobin subunit gamma 1and2 antibody (ET1703-46) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-46) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

ET1703-46_6.jpg

Pic img11 :

Immunohistochemical analysis of paraffin-embedded human stomach tissue (negative) with Rabbit anti-Hemoglobin subunit gamma 1and2 antibody (ET1703-46) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-46) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage