Hemoglobin subunit gamma 1and2 | HUABIO ET1703-46-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Hemoglobin subunit gamma 1and2

catalog :

ET1703-46-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM84-10

reactivity :

human, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Hemoglobin subunit gamma 1and2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human placenta tissue lysate Lane 2: human brain tissue lysate

image 2 :

ICC staining of Hemoglobin subunit gamma 1and2 in D3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-46, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

ICC staining of Hemoglobin subunit gamma 1and2 in MCF-7 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-46, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

ET1703-46-50UL

Target name :

Hemoglobin subunit gamma 1and2

Species reactivity :

Human,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human HBG1 / 2 aa 2-45 / 147.

Uniprot id :

P69891>SwissProt: P69891 Human;SwissProt: P69892 Human

Host :

Rabbit

Clone number :

JM84-10

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human placenta tissue lysate, human brain tissue lysate, human placenta tissue.

Molecular wt :

Predicted band size: 16 kDa

Subcellular location :

Cytosol, hemoglobin complex.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Cell: 1:50-1:100 ;IF-Tissue: 1:50-1:100 ;IHC-P: 1:1000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1703-46_4.jpg

Pic legend4 :

ICC staining of Hemoglobin subunit gamma 1and2 in PC-12 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-46, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1703-46_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Hemoglobin subunit gamma 1and2 antibody (ET1703-46) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-46) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1703-46_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human stomach tissue (negative) with Rabbit anti-Hemoglobin subunit gamma 1and2 antibody (ET1703-46) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-46) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage