CD32 | HUABIO ET1703-43 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD32

catalog :

ET1703-43

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM10-70

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD32 on different lysates with Rabbit anti-CD32 antibody (ET1703-43) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 cell lysate treated with deglycosylation Lane 3: K-562 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 40/31 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-43) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD32 antibody (ET1703-43) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-43) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunocytochemistry analysis of K-562 cells labeling CD32 with Rabbit anti-CD32 antibody (ET1703-43) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD32 antibody (ET1703-43) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

ET1703-43

Target name :

CD32

Species reactivity :

Human

Applications :

WB,IHC-P,IF-Cell,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human CD32 aa 23-274 / 310.

Uniprot id :

P12318>SwissProt: P12318 Human

Host :

Rabbit

Clone number :

JM10-70

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

THP-1 cell lysate, K-562 cell lysate, human spleen tissue, K-562.

Molecular wt :

Predicted band size: 35 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IHC-P: 1:400 ;IF-Cell: 1:100 ;FC: 1:1,000 ;IP: 1-2μg/sample

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1703-43_4.jpg

Pic legend4 :

Flow cytometric analysis of K-562 cells labeling CD32. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1703-43, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1703-43_5.jpg

Pic legend5 :

CD32 was immunoprecipitated from 0.2 mg THP-1 cell lysate with ET1703-43 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1703-43 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 cell lysate (input) Lane 2: ET1703-43 IP in THP-1 cell lysate Lane 3: Rabbit IgG instead of ET1703-43 in THP-1 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 7 seconds; ECL: K1801

more info or order :

HUABIO product webpage