SMC3 | HUABIO ET1703-35 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

SMC3

catalog :

ET1703-35

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM10-75

reactivity :

human, mouse, rat, zebrafish

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of SMC3 on different cells lysates using anti-SMC3 antibody at 1/1,000 dilution. Positive control: Line1: HepG2 cell lysate Line2: NIH-3T3 cell lysate Line3:PC12 cell lysate

image 2 :

ICC staining SMC3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining SMC3 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ET1703-35

Target name :

SMC3

Species reactivity :

Human,Mouse,Rat,Zebrafish

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human SMC3 aa 1994-1217 / 1217.

Uniprot id :

Q9UQE7>SwissProt: Q9UQE7 Human;SwissProt: Q9CW03 Mouse;SwissProt: P97690 Rat

Host :

Rabbit

Clone number :

JM10-75

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2 cell lysate, NIH-3T3 cell lysate, PC12 cell lysate, Hela, HepG2, NIH-3T3, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse colon tissue, zebrafish tissue.

Molecular wt :

Predicted band size: 142 kDa

Subcellular location :

Centromere, Chromosome, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000;IF-Cell: 1:100-1:500;IF-Tissue: 1:100-1:500;IHC-P: 1:50-1:200;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1703-35_4.jpg

Pic legend4 :

ICC staining SMC3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1703-35_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMC3 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1703-35_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SMC3 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1703-35_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SMC3 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1703-35_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SMC3 antibody. Counter stained with hematoxylin.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1703-35_9.jpg

Pic legend9 :

Flow cytometric analysis of Hela cells with SMC3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage