XBP1 | HUABIO ET1703-23 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

XBP1

catalog :

ET1703-23

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM10-62

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of XBP1 on different lysates with Rabbit anti-XBP1 antibody (ET1703-23) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-XBP1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 29 kDa Observed band size: 35 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-23) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of XBP1 on different lysates with Rabbit anti-XBP1 antibody (ET1703-23) at 1/500 dilution. Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 29 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-23) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining XBP1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ET1703-23

Target name :

XBP1

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,IF-Cell,IF-Tissue,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human XBP1 aa 126-175 / 261.

Uniprot id :

P17861>SwissProt: P17861 Human;SwissProt: O35426 Mouse

Host :

Rabbit

Clone number :

JM10-62

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

293T cell lysate, HepG2 cell lysate, Hela, HepG2, SW480, human colon carcinoma tissue, mouse liver tissue, 293T.

Molecular wt :

Predicted band size: 29 kDa

Subcellular location :

Nucleus, Chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1703-23_4.jpg

Pic legend4 :

ICC staining XBP1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1703-23_5.jpg

Pic legend5 :

ICC staining XBP1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1703-23_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-XBP1 antibody (ET1703-23) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-23) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1703-23_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-XBP1 antibody (ET1703-23) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-23) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1703-23_8.jpg

Pic legend8 :

Flow cytometric analysis of 293T cells with XBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage