NOTCH4 | HUABIO ET1703-20 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NOTCH4

catalog :

ET1703-20

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM101-05

reactivity :

mouse

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NOTCH4 on F9 cell lysates with Rabbit anti-NOTCH4 antibody (ET1703-20) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 210 kDa Observed band size: 250 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-20) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

NOTCH4 was immunoprecipitated from 0.2 mg F9 cell lysate with ET1703-20 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1703-20 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: F9 cell lysate (input) Lane 2: ET1703-20 IP in F9 cell lysate Lane 3: Rabbit IgG instead of ET1703-20 in F9 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 59 seconds; ECL: K1802

product information

SKU :

ET1703-20

Target name :

NOTCH4

Species reactivity :

Mouse

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human NOTCH4 aa 1,920-1,960 / 2,003.

Uniprot id :

P31695>SwissProt: P31695 Mouse

Host :

Rabbit

Clone number :

JM101-05

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

F9 cell lysates.

Molecular wt :

Predicted band size: 210 kDa

Subcellular location :

Cell membrane, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IP: 1-2μg/sample

more info or order :

HUABIO product webpage