FGF2 | HUABIO ET1703-18 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

FGF2

catalog :

ET1703-18

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM28-10

reactivity :

human, mouse

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of FGF2 on different lysates with Rabbit anti-FGF2 antibody (ET1703-18) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: U-87 MG cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: 293T cell lysate (20 µg/Lane) Predicted band size: 31 kDa Observed band size: 18/22/24 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-18) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-FGF2 antibody (ET1703-18) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-18) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-FGF2 antibody (ET1703-18) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-18) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1703-18

Target name :

FGF2

Species reactivity :

Human,Mouse

Applications :

WB,IP,FC,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human FGF2 aa 121-170 / 288.

Uniprot id :

P09038>SwissProt: P09038 Human;SwissProt: P15655 Mouse

Host :

Rabbit

Clone number :

JM28-10

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

K-562 cell lysate, U-87 MG cell lysate, HeLa cell lysate, 293T cell lysate, human breast tissue, human prostate tissue, mouse trachea tissue, Jurkat.

Molecular wt :

Predicted band size: 31 kDa

Subcellular location :

Secreted, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IP: 1-2μg/sample ;FC: 1:50-1:100 ;IHC-P: 1:200-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1703-18_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse trachea tissue with Rabbit anti-FGF2 antibody (ET1703-18) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-18) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1703-18_5.jpg

Pic legend5 :

FGF2 was immunoprecipitated from 0.2 mg K-562 cell lysate with ET1703-18 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1703-18 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: K-562 cell lysate (input) Lane 2: ET1703-18 IP in K-562 cell lysate Lane 3: Rabbit IgG instead of ET1703-18 in K-562 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute; ECL: K1802

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1703-18_6.jpg

Pic legend6 :

Flow cytometric analysis of Jurkat cells with FGF2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage