product summary
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company name :
HUABIO
product type :
antibody
product name :
Vitamin D Binding protein
catalog :
ET1703-09
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JM10-36
reactivity :
human
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Vitamin D Binding protein on human plasma lysates with Rabbit anti-Vitamin D Binding protein antibody (ET1703-09) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-09) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Vitamin D Binding protein antibody (ET1703-09) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-09) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
ICC staining of Vitamin D Binding protein in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-09, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
product information
SKU :
ET1703-09
Target name :
Vitamin D Binding protein
Species reactivity :
Human
Applications :
WB,IF-Cell,IF-Tissue,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic pertide within Human Vitamin D Binding protein aa 270-319 / 474.
Uniprot id :
P02774>SwissProt: P02774 Human
Host :
Rabbit
Clone number :
JM10-36
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Human plasma lysates, human pancreas tissue, Hela, HepG2, SKOV-3.
Molecular wt :
Predicted band size: 53 kDa
Subcellular location :
Secreted.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IF-Cell: 1:50-1:200
;IF-Tissue: 1:50-1:200
;IHC-P: 1:50-1:200
;FC: 1:50-1:100
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET1703-09_4.jpg
Pic legend4 :
ICC staining of Vitamin D Binding protein in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-09, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET1703-09_5.jpg
Pic legend5 :
ICC staining of Vitamin D Binding protein in SKOV-3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-09, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ET1703-09_6.jpg
Pic legend6 :
Flow cytometric analysis of Vitamin D Binding protein was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-09, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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