CD90 / THY1 | HUABIO ET1702-92-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD90 / THY1

catalog :

ET1702-92-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JF10-09

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD90 / THY1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: HUVEC cell lysate

image 2 :

All lanes: Western blot analysis of THY1 with anti-CD90 / THY1 antibody[JF10-09] (ET1702-92) at 1:500 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2: THY1 knockdown Hela whole cell lysate (10 µg). ET1702-92 was shown to specifically react with THY1 in wild-type Hela cells. Weakened bands were observed when THY1 knockdown samples were tested. Wild-type and THY1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of HeLa cells labeling CD90 / THY1 with Rabbit anti-CD90 / THY1 antibody (ET1702-92) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD90 / THY1 antibody (ET1702-92) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

ET1702-92-50UL

Target name :

CD90 / THY1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human THY1 aa 75-120 / 161.

Uniprot id :

P04216>SwissProt: P04216 Human;SwissProt: P01831 Mouse;SwissProt: P01830 Rat

Host :

Rabbit

Clone number :

JF10-09

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Hela cell lysate, HepG2 cell lysate, HUVEC cell lysate, human lung carcinoma tissue, human kidney tissue, human tonsil tissue, human spleen tissue, mouse brain tissue, mouse hippocampus tissue.

Molecular wt :

Predicted band size: 18 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:400 ;IHC-P: 1:50-1:200 ;IHC-Fr:1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1702-92_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-CD90 / THY1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-92, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1702-92_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD90 / THY1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-92, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1702-92_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD90 / THY1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-92, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1702-92_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD90 / THY1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-92, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1702-92_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD90 / THY1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-92, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1702-92_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using anti-CD90 / THY1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-92, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1702-92_10.jpg

Pic legend10 :

Immunofluorescence analysis of frozen mouse hippocampus tissue labeling CD90 / THY1 with Rabbit anti-CD90 / THY1 antibody (ET1702-92). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-92, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ET1702-92_11.jpg

Pic legend11 :

Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling CD90 / THY1 with Rabbit anti-CD90 / THY1 antibody (ET1702-92). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-92, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/ET1702-92_12.jpg

Pic legend12 :

Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling CD90 / THY1 with Rabbit anti-CD90 / THY1 antibody (ET1702-92) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-92, green) at 1/400 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img13 :

https://storage.huabio.cn/huabio/productImg/ET1702-92_13.jpg

Pic legend13 :

Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling CD90 / THY1 with Rabbit anti-CD90 / THY1 antibody (ET1702-92) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-92, green) at 1/400 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage