alpha 1 Antitrypsin | HUABIO ET1702-87 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

alpha 1 Antitrypsin

catalog :

ET1702-87

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JF10-03

reactivity :

human

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of alpha 1 Antitrypsin on human spleen tissue lysates with Rabbit anti-alpha 1 Antitrypsin antibody (ET1702-87) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-87) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining alpha 1 Antitrypsin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining alpha 1 Antitrypsin in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ET1702-87

Target name :

alpha 1 Antitrypsin

Species reactivity :

Human

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human alpha 1 Antitrypsin aa 1-418 / 418.

Uniprot id :

P01009>SwissProt: P01009 Human

Host :

Rabbit

Clone number :

JF10-03

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human spleen tissue lysates, MCF-7, Hela, HepG2, human spleen tissue, human liver tissue, human breast carcinoma tissue.

Molecular wt :

Predicted band size: 47 kDa

Subcellular location :

Secreted, Endoplasmic reticulum.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IHC-P: 1:50-1:200;IP: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1702-87_4.jpg

Pic legend4 :

ICC staining alpha 1 Antitrypsin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1702-87_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-alpha 1 Antitrypsin antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1702-87_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-alpha 1 Antitrypsin antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1702-87_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-alpha 1 Antitrypsin antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage