p38 alpha / MAPK14 | HUABIO ET1702-65 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

p38 alpha / MAPK14

catalog :

ET1702-65

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JF55-07

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of p38 alpha / MAPK14 on different lysates with Rabbit anti-p38 alpha / MAPK14 antibody (ET1702-65) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HEK-293 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: Neuro-2a cell lysate Lane 6: RAW264.7 cell lysate Lane 7: PC-12 cell lysate Lane 8: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-65) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

All lanes: Western blot analysis of p38 alpha / MAPK14 with anti-p38 alpha / MAPK14 antibody[JF55-07] (ET1702-65) at 1:500 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2: p38 alpha / MAPK14 knockout Hela whole cell lysate (10 µg). ET1702-65 was shown to specifically react with p38 alpha / MAPK14 in wild-type Hela cells. No band was observed when p38 alpha / MAPK14 knockout sample was tested. Wild-type and p38 alpha / MAPK14 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-65, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of p38 alpha / MAPK14 on different lysates with Rabbit anti-p38 alpha / MAPK14 antibody (ET1702-65) at 1/1,000 dilution. Lane 1: THP-1 cell lysate (10 µg/Lane) Lane 2: C2C12 cell lysate (10 µg/Lane) Lane 3: PC-12 cell lysate (10 µg/Lane) Lane 4: Rat kidney tissue lysate (20 µg/Lane) Lane 5: Mouse kidney tissue lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 3 minutes 10 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-65) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1702-65

Target name :

p38 alpha / MAPK14

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within C-terminal human MAPK14.

Uniprot id :

Q16539>SwissProt: Q16539 Human;SwissProt: P47811 Mouse;SwissProt: P70618 Rat

Host :

Rabbit

Clone number :

JF55-07

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, C6 cell lysate, THP-1 cell lysate, C2C12 cell lysate, Rat kidney tissue lysate, Mouse kidney tissue lysate, RAW264.7, PC-12, HeLa, mouse heart tissue.

Molecular wt :

Predicted band size: 41 kDa

Subcellular location :

Nucleus, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:1,000

Advanced Validation :

Knockout (KO)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1702-65_4.jpg

Pic legend4 :

Immunocytochemistry analysis of RAW264.7 cells labeling p38 alpha / MAPK14 with Rabbit anti-p38 alpha / MAPK14 antibody (ET1702-65) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 alpha / MAPK14 antibody (ET1702-65) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1702-65_5.jpg

Pic legend5 :

Immunocytochemistry analysis of PC-12 cells labeling p38 alpha / MAPK14 with Rabbit anti-p38 alpha / MAPK14 antibody (ET1702-65) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 alpha / MAPK14 antibody (ET1702-65) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1702-65_6.jpg

Pic legend6 :

Flow cytometric analysis of RAW264.7 cells labeling p38 alpha / MAPK14. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-65, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1702-65_7.jpg

Pic legend7 :

Flow cytometric analysis of HeLa cells labeling p38 alpha / MAPK14. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-65, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1702-65_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-p38 alpha / MAPK14 antibody (ET1702-65) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-65) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage