Glucagon | HUABIO ET1702-59 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Glucagon

catalog :

ET1702-59

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JF33-10

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunofluorescence analysis of paraffin-embedded mouse pancreas tissue labeling Glucagon with Rabbit anti-Glucagon antibody (ET1702-59) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-59, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

image 2 :

ICC staining GLP1 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining GLP1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ET1702-59

Target name :

Glucagon

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human GLP1 aa 65-150.

Uniprot id :

P01275>SwissProt: P01275 Human;SwissProt: P55095 Mouse;SwissProt: P06883 Rat

Host :

Rabbit

Clone number :

JF33-10

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

PC-12, SW480, human pancreas tissue.

Molecular wt :

Predicted band size: 21 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB:1:500-1:1,000 ;IF-Cell:1:50-1:200 ;IF-Tissue:1:100 ;IHC-P:1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1702-59_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GLP1 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage