TGN46 | HUABIO ET1702-56 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TGN46

catalog :

ET1702-56

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JF1-024

reactivity :

human, mouse

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TGN46 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-56, 1/1,000) was used in 5% NFDM/TBST at 4° overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate

image 2 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGN46 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-TGN46 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-56, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1702-56

Target name :

TGN46

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,IP,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human TGN46 aa 43-79 / 437L5L562:L576.

Uniprot id :

O43493>SwissProt: O43493 Human;SwissProt: Q62314 Mouse

Host :

Rabbit

Clone number :

JF1-024

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

NIH/3T3 cell lysate, Hela cell lysate, human kidney tissue, human liver tissue.

Molecular wt :

95 kDa

Subcellular location :

Cell membrane, Golgi apparatus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IP: 1:50-1:100 ;IHC-P: 1:50-1:200 ;IF-Cell: 1:100-1:200 ;FC: 1:1000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1702-56_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-TGN46 antibody (ET1702-56) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-56) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1702-56_5.jpg

Pic legend5 :

Immunocytochemistry analysis of HepG2 cells labeling TGN46 with Rabbit anti-TGN46 antibody (ET1702-56) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TGN46 antibody (ET1702-56) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1702-56_6.jpg

Pic legend6 :

Flow cytometric analysis of HepG2 cells labeling TGN46. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-56, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1702-56_7.png

Pic legend7 :

Application: IF-cell Species: Human Sample: HeLa, U2OS, RPE1 Antibody concentration: 1: 200 (red letter) Date by conrtesy of: Dr. Yongjia Wang, College of Biological Sciences, China agricultural University

more info or order :

HUABIO product webpage