PDGFR alpha | HUABIO ET1702-49-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PDGFR alpha

catalog :

ET1702-49-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JF104-6

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: E14.5 embyro Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse embryonic femur tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-49) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse embryonic intervertebral disc tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-49) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1702-49-50UL

Target name :

PDGFR alpha

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within C-terminal human PDGFR alpha.

Uniprot id :

P16234>SwissProt: P16234 Human;SwissProt: P26618 Mouse;SwissProt: P20786 Rat

Host :

Rabbit

Clone number :

JF104-6

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse embryonic femur tissue, mouse embryonic intervertebral disc tissue, mouse embryonic lung tissue, rat embryonic femur tissue, rat embryonic intervertebral disc tissue, rat embryonic lung tissue, rat endometrium tissue, human colon tissue, human colon cancer tissue, human glioblastoma tissue, NIH/3T3 cell lysates, SHG-44 cell lysates, A549, NIH/3T3.

Molecular wt :

Predicted band size: 123 kDa

Subcellular location :

Golgi apparatus, Cell membrane, cilium.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000-1:10,000 ;IHC-P: 1:500-1:2,000 ;IHC-Fr: 1:500

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1702-49_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse embryonic lung tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-49) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1702-49_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat embryonic femur tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-49) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1702-49_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat embryonic intervertebral disc tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-49) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1702-49_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat embryonic lung tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-49) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1702-49_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat endometrium tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-49) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1702-49_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-49) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1702-49_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-49) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ET1702-49_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded human glioblastoma tissue with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-49) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/ET1702-49_12.jpg

Pic legend12 :

Western blot analysis of PDGFR alpha on NIH/3T3 cell lysates with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 123 kDa Observed band size: 180 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-49) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img13 :

https://storage.huabio.cn/huabio/productImg/ET1702-49_13.jpg

Pic legend13 :

Western blot analysis of PDGFR alpha on different lysates with Rabbit anti-PDGFR alpha antibody (ET1702-49) at 1/5,000 dilution. Lane 1: NIH/3T3-si NT cell lysate Lane 2: NIH/3T3-si PDGFR alpha cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 123 kDa Observed band size: 190 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-49, 1/5,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage