product summary
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company name :
HUABIO
product type :
antibody
product name :
Phospho-Smad2 (S255)
catalog :
ET1702-34
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JF0882
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO ET1702-34 image 1
Western blot analysis of Phospho-Smad2 (S255) on different lysates with Rabbit anti-Phospho-Smad2 (S255) antibody (ET1702-34) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 200nM Calyculin A for 1 hour cell lysate Lane 3: HeLa treated with 200nM Calyculin A for 1 hour cell lysate, then the membrane treated with λpp for 1 hour Lane 4: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 60 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-34) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO ET1702-34 image 2
Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Phospho-Smad2 (S255) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-34, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
ET1702-34
Target name :
Phospho-Smad2 (S255)
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser255 of Human Smad2 aa 229-272 / 467.
Uniprot id :
Q15796>SwissProt: Q15796 Human;SwissProt: Q62432 Mouse;SwissProt: O70436 Rat
Host :
Rabbit
Clone number :
JF0882
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, HeLa treated with 200nM Calyculin A for 1 hour cell lysate, RAW264.7 cell lysate, mouse uterus tissue.
Molecular wt :
Predicted band size: 52 kDa
Subcellular location :
Nucleus, Cytoplasm.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.
Advanced Validation :
Cell treatment (CT)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!