LYVE1 | HUABIO ET1702-29-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

LYVE1

catalog :

ET1702-29-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JF0979

reactivity :

human

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of LYVE1 on different lysates with Rabbit anti-LYVE1 antibody (ET1702-29) at 1/2,000 dilution. Lane 1: MCF-7 cell lysate Lane 2: SW480 cell lysate Lane 3: A431 cell lysate Lane 4: HUVEC cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-29) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of LYVE1 on different lysates with Rabbit anti-LYVE1 antibody (ET1702-29) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si LYVE1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-29, 1/2,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1702-29-50UL

Target name :

LYVE1

Species reactivity :

Human

Applications :

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human LYVE1 aa 36-72 / 322.

Uniprot id :

Q9Y5Y7>SwissProt: Q9Y5Y7 Human

Host :

Rabbit

Clone number :

JF0979

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MCF-7 cell lysate, SW480 cell lysate, A431 cell lysate, HUVEC cell lysate, A431, HUVEC, SW480.

Molecular wt :

Predicted band size: 35 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000

Advanced Validation :

Knockdown (KD)

more info or order :

HUABIO product webpage