antibody

Anti-Progesterone Receptor Antibody [JF0549]

ET1702-24TR

20 uL

99 USD

monoclonal

domestic rabbit

nonconjugated

JF0549

western blot, immunoprecipitation, immunohistochemistry - paraffin section

Anti-Progesterone Receptor Antibody [JF0549]

Human

WB,IF-Cell,IF-Tissue,IHC-P,IP

Non-conjugated

Synthetic peptide within N-terminal human Progesterone Receptor.

SwissProt: P06401 Human

Rabbit

JF0549

IgG

20 uL

99 USD

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Liquid

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Protein A affinity purified.

Recombinant Rabbit monoclonal Antibody

MCF-7, human breast tissue, human cervix tissue, human uterus tissue, human smooth muscle tissue.

99 kDa

Nucleus, Cytoplasm, Mitochondrion outer membrane.

1 mg/mL.

WB: 1:1,000 ;IF-Cell: 1:50-1:100 ;IF-Tissue: 1:50-1:100 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.

ET1702-24_2.jpg

Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Progesterone Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1702-24_3.jpg

Immunohistochemical analysis of paraffin-embedded human cervix tissue using anti-Progesterone Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1702-24_4.jpg

Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Progesterone Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1702-24_5.jpg

Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Progesterone Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-24, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1702-24_6.jpg

Immunohistochemical analysis of paraffin-embedded human smooth muscle tissue using anti-Progesterone Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-24, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.