Glutathione Peroxidase 1 | HUABIO ET1702-09 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Glutathione Peroxidase 1

catalog :

ET1702-09

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JF0944

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Glutathione Peroxidase 1 on different lysates with Rabbit anti-Glutathione Peroxidase 1 antibody (ET1702-09) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: THP-1 cell lysate Lane 3: HEK-293 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-09) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Glutathione Peroxidase 1 antibody (ET1702-09) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-09) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.

product information

SKU :

ET1702-09

Target name :

Glutathione Peroxidase 1

Species reactivity :

Human,Mouse

Applications :

WB,IF-Tissue,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Glutathione Peroxidase 1 aa 1-50 / 203.

Uniprot id :

P07203>SwissProt: P07203 Human;SwissProt: P11352 Mouse

Host :

Rabbit

Clone number :

JF0944

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SH-SY5Y cell lysate, THP-1 cell lysate, HEK-293 cell lysate, human liver tissue, human kidney tissue, mouse colon tissue, human tonsil tissue, human breast carcinoma tissue.

Molecular wt :

Predicted band size: 22 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1702-09_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1702-09_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1702-09_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage