product summary
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company name :
HUABIO
product type :
antibody
product name :
ABL2
catalog :
ET1702-07
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JF0951
reactivity :
human, mouse, rat
application :
western blot, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of ABL2 on different lysates with Rabbit anti-ABL2 antibody (ET1702-07) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-ABL2 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 128 kDa
Observed band size: 128 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-07) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of ABL2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-07, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Jurkat cell lysate
Predicted band size: 128 kDa
Observed band size: 128 kDa
image 3 :
Flow cytometric analysis of ABL2 was done on Raji cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-07, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
ET1702-07
Target name :
ABL2
Species reactivity :
Human,Rat,Mouse
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human ABL2 aa 502-552 / 1182.
Uniprot id :
P42684>SwissProt: P42684 Human;SwissProt: Q4JIM5 Mouse;Unigene: 69847 Rat
Host :
Rabbit
Clone number :
JF0951
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Hela cell lysate, PC-12 cell lysate, Jurkat cell lysate, Raji.
Molecular wt :
Predicted band size: 128 kDa
Subcellular location :
Cytoplasm, cytoskeleton.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000
;FC: 1:50-1:100
Advanced Validation :
Knockdown (KD)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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