Transferrin Receptor (CD71) | HUABIO ET1702-06-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Transferrin Receptor (CD71)

catalog :

ET1702-06-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JF0956

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (ET1702-06) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: SW480 cell lysate Lane 4: U-87 MG cell lysate Lane 5: RAW264.7 cell lysate Lane 6: Mouse spleen tissue lysate Lane 7: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-06) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (ET1702-06) at 1/1,000 dilution. Lane 1: HeLa-si NT cell lysate Lane 2: HeLa-si Transferrin Receptor cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-06) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (ET1702-06) at 1/5,000 dilution. Lane 1: HAP1 WT cell lysate Lane 2: HAP1 Transferrin Receptor KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 85 kDa Observed band size: 90 kDa Exposure time: 1 minute 48 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-06) at 1/5,000 dilution was used in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1702-06-50UL

Target name :

Transferrin Receptor (CD71)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human CD71 aa 22-60 / 760.

Uniprot id :

P02786>SwissProt: P02786 Human;SwissProt: Q62351 Mouse;SwissProt: Q99376 Rat

Host :

Rabbit

Clone number :

JF0956

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, K-562 cell lysate, SW480 cell lysate, U-87 MG cell lysate, RAW264.7 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, Hela.

Molecular wt :

Predicted band size: 85 kDa

Subcellular location :

Cell membrane, Melanosome, Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1702-06_4.jpg

Pic legend4 :

Flow cytometric analysis of HeLa cells labeling Transferrin Receptor (CD71). Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-06, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage