Mannose Receptor(CD206) | HUABIO ET1702-04-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Mannose Receptor(CD206)

catalog :

ET1702-04-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JF0953

reactivity :

human, mouse

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Mannose Receptor(CD206) on different lysates with Rabbit anti-Mannose Receptor(CD206) antibody (ET1702-04) at 1/5,000 dilution. Lane 1: MOLT-4 cell lysate (20 µg/Lane) Lane 2: Mouse lung tissue lysate (40 µg/Lane) Predicted band size: 166 kDa Observed band size: 166 kDa Exposure time: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-04) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

All lanes: Western blot analysis of CD206 with anti-CD206 antibody[JF0953] (ET1702-04) at 1:1,000 dilution. Lane 1: Wild-type HepG2 whole cell lysate (10 µg). Lane 2: CD206 knockdown HepG2 whole cell lysate (10 µg). Lane 3: CD206 knockdown HepG2 whole cell lysate (10 µg). ET1702-04 was shown to specifically react with CD206 in wild-type Hela cells. Weakened bands were observed when CD206 knockdown samples were tested. Wild-type and CD206 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-04, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of Mannose Receptor(CD206) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-04, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human lung tissue lysate Lane 2: HepG2 cell lysate

product information

SKU :

ET1702-04-50UL

Target name :

Mannose Receptor(CD206)

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human CD206 aa 1,407-1,456 / 1,456.

Uniprot id :

P22897>SwissProt: P22897 Human;SwissProt: Q61830 Mouse

Host :

Rabbit

Clone number :

JF0953

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MOLT-4 cell lysate, Mouse lung tissue lysate, Human lung tissue lysate, HepG2 cell lysate, D3 cell lysate, HepG2.

Molecular wt :

Predicted band size: 166 kDa

Subcellular location :

Endosome membrane, Cell membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Cell: 1:100 ;FC: 1:500-1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1702-04_4.jpg

Pic legend4 :

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1702-04_5.jpg

Pic legend5 :

Immunocytochemistry analysis of HepG2 cells labeling Mannose Receptor(CD206) with Rabbit anti-Mannose Receptor(CD206) antibody (ET1702-04) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Mannose Receptor(CD206) antibody (ET1702-04) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1702-04_6.jpg

Pic legend6 :

Flow cytometric analysis of HepG2 cells labeling Mannose Receptor(CD206). Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-04, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage