Doublecortin | HUABIO ET1701-98 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Doublecortin

catalog :

ET1701-98

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JJ0959

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Rat Site: Hippocampus Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 3 :

Western blot analysis of Doublecortin on different lysates with Rabbit anti-Doublecortin antibody (ET1701-98) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate (20 µg/Lane) Lane 2: Human brain tissue lysate (40 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 41 kDa Observed band size: 41/45 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-98) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1701-98

Target name :

Doublecortin

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Doublecortin aa 22-71 / 365.

Uniprot id :

O43602>SwissProt: O43602 Human;SwissProt: O88809 Mouse;SwissProt: Q9ESI7 Rat

Host :

Rabbit

Clone number :

JJ0959

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Neuro-2a cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, HepG2.

Molecular wt :

Predicted band size: 41 kDa

Subcellular location :

Cytoplasm, Cell projection, neuron projection.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IHC-P: 1:1,000 ;IHC-Fr: 1:500 ;IF-Tissue: 1:200-1:500

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1701-98_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Doublecortin antibody (ET1701-98) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-98) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1701-98_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-Doublecortin antibody (ET1701-98) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-98) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1701-98_6.jpg

Pic legend6 :

Application: IF-Tissue Species: Mouse Site: Hippocampus Sample: Paraffin-embedded section Antibody concentration: 1/500

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1701-98_7.jpg

Pic legend7 :

Application: IF-Tissue Species: Rat Site: Hippocampus Sample: Paraffin-embedded section Antibody concentration: 1/500

more info or order :

HUABIO product webpage