Insulin degrading enzyme | HUABIO ET1701-97 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Insulin degrading enzyme

catalog :

ET1701-97

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JJ0949

reactivity :

human, mouse, rat, zebrafish

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Insulin degrading enzyme on different lysates with Rabbit anti-Insulin degrading enzyme antibody (ET1701-97) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Insulin degrading enzyme KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 118 kDa Observed band size: 100 kDa Exposure time: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-97) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Insulin degrading enzyme on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-97, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Zebrafish tissue lysate Lane 2: Hela cell lysate Lane 3: K562 cell lysate

image 3 :

Western blot analysis of Insulin degrading enzyme on hybrid fish (crucian-carp) heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-97, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1701-97

Target name :

Insulin degrading enzyme

Species reactivity :

Human,Mouse,Rat,Zebrafish

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Insulin degrading enzyme aa 1-106 / 1019.

Uniprot id :

P14735>SwissProt: P14735 Human;SwissProt: Q9JHR7 Mouse;SwissProt: P35559 Rat

Host :

Rabbit

Clone number :

JJ0949

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Zebrafish tissue lysate, Hela cell lysate, K562 cell lysate, human liver carcinoma tissue, human colon carcinoma tissue, human stomach carcinoma tissue, mouse colon tissue, hybrid fish (crucian-carp) heart tissue lysates.

Molecular wt :

Predicted band size: 118 kDa

Subcellular location :

Cytoplasm, Cell membrane, Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:50-1:200

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1701-97_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Insulin degrading enzyme antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-97, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1701-97_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Insulin degrading enzyme antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-97, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1701-97_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Insulin degrading enzyme antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-97, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1701-97_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Insulin degrading enzyme antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-97, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage