product summary
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company name :
HUABIO
product type :
antibody
product name :
RIP
catalog :
ET1701-79
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JJ092-04
reactivity :
human
application :
western blot, immunoprecipitation, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of RIP on different lysates with Rabbit anti-RIP antibody (ET1701-79) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Raji cell lysate
Lane 3: K-562 cell lysate
Lane 4: 293T cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 76 kDa
Observed band size: 75 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-79) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Flow cytometric analysis of HeLa cells labeling RIP.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1701-79, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
image 3 :
RIP was immunoprecipitated from 0.2 mg HeLa cell lysate with ET1701-79 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1701-79 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: ET1701-79 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of ET1701-79 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 2 minutes; ECL: K1801
product information
SKU :
ET1701-79
Target name :
RIP
Species reactivity :
Human
Applications :
WB,FC,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human RIP aa 250-500.
Uniprot id :
Q13546>SwissProt: Q13546 Human
Host :
Rabbit
Clone number :
JJ092-04
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysates, Raji cell lysates, 293.
Molecular wt :
Predicted band size: 76 kDa
Subcellular location :
Cell membrane, Cytoplasm, Membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;FC: 1:1,000
;IP: 1-2μg/sample
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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