product summary
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company name :
HUABIO
product type :
antibody
product name :
Anti-LC3B Antibody [JJ090-6]
catalog :
ET1701-65TR
quantity :
20 uL
price :
99 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JJ090-6
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation
more info or order :
HUABIO product webpage
product information
SKU :
ET1701-65TR
Target name :
Anti-LC3B Antibody [JJ090-6]
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IP,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human LC3 B aa 1-20 and aa 80-120.
Uniprot id :
SwissProt: Q9GZQ8 Human;SwissProt: Q9CQV6 Mouse;SwissProt: Q62625 Rat
Host :
Rabbit
Clone number :
JJ090-6
Isotype :
IgG
Size :
20 uL
List Price :
99 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HCT 116 cell lysate, HCT 116 treated with 50μM Chloroquine for 18 hours cell lysate, U-87 MG cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, HeLa cells treated with 50μM Chloroquine for 24 hours.
Molecular wt :
Predicted band size: 14/16 kDa
Subcellular location :
Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule, Mitochondrion.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000 ;IP: Use at an assay dependent concentration. ;IF-Cell: 1:200
Pic img4 :
ET1701-65_2.jpg
Pic img5 :
Western blot analysis of LC3B on different lysates with Rabbit anti-LC3B antibody (ET1701-65) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50μM Chloroquine for 18 hours cell lysate Lane 3: C2C12 cell lysate Lane 4: C2C12 treated with 50μM Chloroquine for 18 hours cell lysate Lane 5: C6 cell lysate Lane 6: C6 treated with 50μM Chloroquine for 18 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 14/16 kDa Observed band size: 14/16 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-65) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Pic legend5 :
ET1701-65_3.jpg
Pic legend6 :
Western blot analysis of LC3B on different lysates with Rabbit anti-LC3B antibody (ET1701-65) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HCT 116 treated with 50μM Chloroquine for 18 hours cell lysate Lane 3: U-87 MG cell lysate Lane 4: C6 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 14/16 kDa Observed band size: 14/16 kDa Exposure time: 26 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-65) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!