SUZ12 | HUABIO ET1701-62 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

SUZ12

catalog :

ET1701-62

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JJ09-04

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of SUZ12 on different lysates with Rabbit anti-SUZ12 antibody (ET1701-62) at 1/500 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: PC-12 cell lysate Lane 3: Raw264.7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-62) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of SUZ12 on MCF-7 cells lysates using anti-SUZ12 antibody at 1/1,000 dilution.

image 3 :

IF-cell staining SUZ12 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ET1701-62

Target name :

SUZ12

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human SUZ12 aa 1-150/739.

Uniprot id :

Q15022>SwissProt: Q15022 Human;SwissProt: Q80U70 Mouse;Entrez Gene: 688041 Rat

Host :

Rabbit

Clone number :

JJ09-04

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MCF-7 cells lysates, NIH/3T3 cell lysate, PC-12 cell lysate, Raw264.7 cell lysate, SW480, MCF-7, Hela, SKOV-3.

Molecular wt :

Predicted band size: 83 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IP: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1701-62_4.jpg

Pic legend4 :

IF-cell staining SUZ12 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1701-62_5.jpg

Pic legend5 :

IF-cell staining SUZ12 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1701-62_6.jpg

Pic legend6 :

IF-cell staining SUZ12 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

more info or order :

HUABIO product webpage