product summary
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company name :
HUABIO
product type :
antibody
product name :
KMT6 / EZH2
catalog :
ET1701-56
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JJ089-9
reactivity :
human, mouse
application :
chromatin immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-KMT6 / EZH2 antibody (ET1701-56) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-56) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 2 :
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-KMT6 / EZH2 antibody (ET1701-56) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-56) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-KMT6 / EZH2 antibody (ET1701-56) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-56) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
ET1701-56
Target name :
KMT6 / EZH2
Species reactivity :
Human,Mouse
Applications :
IHC-P,IF-Cell,IF-Tissue,ChIP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human EZH2 aa 140-290.
Uniprot id :
Q15910>SwissProt: Q15910 Human;SwissProt: Q61188 Mouse
Host :
Rabbit
Clone number :
JJ089-9
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Human breast cancer tissue, human colon cancer tissue, human tonsil tissue, human NK T-Cell lymphoma tissue, HeLa, Neuro-2a.
Molecular wt :
Predicted band size: 85 kDa
Subcellular location :
Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
IHC-P: 1:500
;IF-Cell: 1:100
;IF-Tissue: 1:50-1:200
;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET1701-56_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human NK T-Cell lymphoma tissue with Rabbit anti-KMT6 / EZH2 antibody (ET1701-56) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-56) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET1701-56_5.jpg
Pic legend5 :
Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with KMT6 / EZH2 (ET1701-56) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ET1701-56_6.jpg
Pic legend6 :
Immunocytochemistry analysis of HeLa cells labeling KMT6 / EZH2 with Rabbit anti-KMT6 / EZH2 antibody (ET1701-56) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KMT6 / EZH2 antibody (ET1701-56) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/ET1701-56_7.jpg
Pic legend7 :
Immunocytochemistry analysis of Neuro-2a cells labeling KMT6 / EZH2 with Rabbit anti-KMT6 / EZH2 antibody (ET1701-56) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KMT6 / EZH2 antibody (ET1701-56) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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