Caspase-10 | HUABIO ET1701-53 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Caspase-10

catalog :

ET1701-53

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JJ0890

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Caspase-10 on different lysates with Rabbit anti-Caspase-10 antibody (ET1701-53) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: HepG2 cell lysate Lane 4: A431 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 59 kDa Observed band size: 59/58 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-53) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of Jurkat cells labeling Caspase-10 with Rabbit anti-Caspase-10 antibody (ET1701-53) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-10 antibody (ET1701-53) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Caspase-10 antibody (ET1701-53) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-53) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1701-53

Target name :

Caspase-10

Species reactivity :

Human

Applications :

WB,IHC-P,FC,IP,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Caspase-10 aa 254-452 / 521.

Uniprot id :

Q92851>SwissProt: Q92851 Human

Host :

Rabbit

Clone number :

JJ0890

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysate, K-562 cell lysate, HepG2 cell lysate, A431 cell lysate, Jurkat, human kidney tissue, human liver tissue.

Molecular wt :

Predicted band size: 59 kDa

Subcellular location :

Cytosol

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;FC: 1:1,000 ;IHC-P: 1:50-1:200 ;IP: 1-2μg/sample ;IF-Cell: 1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1701-53_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Caspase-10 antibody (ET1701-53) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-53) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1701-53_5.jpg

Pic legend5 :

Flow cytometric analysis of Jurkat cells labeling Caspase-10. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1701-53, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1701-53_6.jpg

Pic legend6 :

Caspase-10 was immunoprecipitated from 0.2 mg K-562 cell lysate with ET1701-53 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1701-53 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: K-562 cell lysate (input) Lane 2: ET1701-53 IP in K-562 cell lysate Lane 3: Rabbit IgG instead of ET1701-53 in K-562 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 7 seconds; ECL: K1802

more info or order :

HUABIO product webpage