Anti-PAX8 Antibody [JJ08-88] | HUABIO ET1701-50TR product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Anti-PAX8 Antibody [JJ08-88]

catalog :

ET1701-50TR

quantity :

20 uL

price :

99 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JJ08-88

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

product information

SKU :

ET1701-50TR

Target name :

Anti-PAX8 Antibody [JJ08-88]

Species reactivity :

Human

Applications :

WB,IF-Cell,IF-Tissue,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human PAX8 aa 101-450 / 450.

Uniprot id :

SwissProt: Q06710 Human

Host :

Rabbit

Clone number :

JJ08-88

Isotype :

IgG

Size :

20 uL

List Price :

99 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SKOV-3 cell lysates, SKOV-3, human thyroid tissue, human ovarian carcinoma tissue, human thyroid cancer tissue, human kidney tissue.

Molecular wt :

Predicted band size: 48 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:500

Pic img4 :

ET1701-50_2.jpg

Pic img5 :

ICC staining of PAX8 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-50, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic legend5 :

ET1701-50_3.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-PAX8 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-50, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

ET1701-50_4.jpg

Pic img8 :

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue with Rabbit anti-PAX8 antibody (ET1701-50) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-50) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic legend8 :

ET1701-50_5.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue using anti-PAX8 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-50, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

ET1701-50_6.jpg

Pic img11 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PAX8 antibody (ET1701-50) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-50) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage